Biocurious is a weblog about biology, quantified.

Hot off the Press: Quantum Dot DNA Sensors

by PhilipJ on 10 November 2005

In the most recent issue of Nature Materials, a Letter describing a new quantum dot(QD)-based DNA nanosensor is presented, a technique that is entirely different from traditional microarray chips.

Due to the unique properties of QDs (see this wikipedia entry), a group from Johns Hopkins has found a way to label individual quantum dots with many copies of a short probe strand of DNA. Upon binding a dye-labelled reporter strand of DNA, the quantum dot and dyed reporter strand act as an acceptor-donor pair for fluorescence resonsance energy transfer—FRET—based detection.

Without bound reporter strands, background noise is near-zero, while a detectable signal is seen for as few as 50 bound reporter molecules to a single QD. As a proof-of-concept, they also report on detection of a reporter molecule with a single point mutations, typical of some ovarian tumors.

The full article is here, while Nature’s own News and Views article is here (subscription required for both).



  1. libero login    95 days ago    #

    On libero’s front web page, there are a number of methods you could opt to reach libero mail. You could choose the mail food selection on the appropriate side of the web page or pick one at the end of the web page.


Name
Email
http://
Message
  Textile help